STATION 23. Examine the four gels in which 20 human hemoglobin samples have been loaded and run. The furthest sample to the right was a control lacking a hemoglobin sample. The loading dye shows up as blue. The A hemoglobin derived from the A allele is less mobile in a gel than the B hemoblobin.

A) Allele frequency of A in this population of 20?

B) Genotype frequency of AB in this population of 20?

C) Genotype frequency of BB in this population of 20?

D) Is this population at Hardy-Weinberg equilibrium?

Answers: A) 0.50 or 50% (20 A alleles/40 total alleles)  B) 0.50 or 50% (10 AB individuals/20 total individuals)  C) 0.25 or 25% (5 BB individuals/20 total individuals)  D) yes (Given the allele frequencies of a = 0.5 and b = 0.5, you would predict genotype frequencies of AA = 0.25, AB = 0.5, and BB = 0.25. The predicted genotype frequencies math the observed genotype frequencies. Thus the population is at Hardy Weinberg Equilibrium.)